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A cell based assay for evaluating binding and uptake of an
WebJul 13, 2024 · Finally, the exclusive binding specificity of the TSAxCD3 bsAbs to CD3 and their tumor antigen was assessed by flow cytometry, and no off-target binding was observed on the various negative cell ... WebCell-based binding assay (CBA), thereby is required by most regulatory guidelines as a further complement of LBA for determining the mechanism of action and bioactivity of therapeutic antibodies. In contrast to LBA, CBA employs native cellular antigens, no need for synthesizing and purifying recombinant ligands. WebCompared to radioligand methods, flow cytometry offers the advantage of monitoring ligand binding to single cells in real time without the need to separate bound from unbound ligands. These binding data can be collected on a microsecond time scale and, because single-cell fluorescence is measured, the heterogeneity in the binding properties of ... flag in thailand