Elf5-cre

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August undefined, 2024

WebApr 20, 2012 · The Elf5-null mice have been described previously . Mice of C57BL/6 strain (6–8 generations) were used for experiments with virgin mammary glands. The genotype … WebG@ Bð% Áÿ ÿ ü€ H FFmpeg Service01w ...

Elf5 MGI Mouse Gene Detail - MGI:1335079 - E74-like factor 5

WebOct 5, 2008 · Epigenetic silencing of Elf5 by DNA methylation interrupts this trophoblast-specific reinforcement loop in the embryonic lineage, and thereby safeguards embryonic … WebApr 19, 2024 · Moreover, haploinsufficiency of Elf5 limits the use of current mouse models to investigate adult tissue distribution of Elf5. Here, we successfully generated Elf5 Cre ERT 2- GFP bacterial artificial chromosome (BAC) transgenic mice and show that Elf5 + cells are present in several adult tissues, where its expression was previously not known. eju8542

ELF5 inhibits the proliferation and invasion of breast …

WebSep 10, 2010 · To further probe the effects of loss of Elf5 during pregnancy, we compared mammary glands from K14-Cre/Elf5 f/f animals and their wildtype type littermate controls … WebMar 13, 2024 · For this purpose, we generate a transgenic mouse with the Cre recombinase inserted into the endogenous locus of Elf5 gene that is expressed specifically in … WebOct 21, 2012 · The genotype of the control WT animals was either K14-Cre/Elf5 +/+ or Elf5 f/f whereas Elf5 conditional knockouts (referred to as Elf5-KO) were K14-Cre/Elf5 f/f. Animal procedures were conducted ... eju8332

Acetylation of ELF5 suppresses breast cancer progression by

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WebMay 15, 2009 · However, the Elf5 in K14-Cre/Elf5 +/f mammary tissue was expressed in a mosaic pattern (Fig. 6 E) and complete ablation of Elf5 expression was confirmed in K14-Cre/Elf5 f/f mammary epithelium (Fig. 6 I). To determine whether alterations in PrlR/Jak2/Stat5 signaling pathway are responsible for the failed lobuloalveolar … WebTherefore, in mice (Elf5-Cre knock-in), the expression of Cre recombinase driven by the Elf5 promoter can be used to study genes specifically and efficiently in the trophoblast lineage during ... teadvushäire tunnusedWeb建立和稳定了包括人滋养层干细胞系、早期及足月人胎盘原代滋养层细胞、滋养层细胞-血管内皮细胞三维共培养、蜕膜dNK细胞等体外研究模型，并稳定了CYP19-Cre、ELF5-Cre介导或慢病毒/腺病毒介导的胎盘特异基因 … eju8583

"WebATP6V1B1 Antibody (PA5-56878) in ICC/IF. Immunofluorescence analysis of V-ATPase B1 was performed using 70% confluent log phase HaCaT and BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. " - Elf5-cre

Elf5-cre

WebOct 5, 2008 · Epigenetic silencing of Elf5 by DNA methylation interrupts this trophoblast-specific reinforcement loop in the embryonic lineage, and thereby safeguards embryonic cells from differentiating into ... WebApr 1, 2024 · The HoxB7->Cre transgenic line efficiently inactivates RBPJ in the ureteric bud lineage by E14.5 A. RBPJ f/f (wild-type) E14.5 littermate kidneys express RBPJ (green) in all cells including ...

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WebReal-time RT–PCR analyses demonstrated that the levels of Elf5 mRNA were lower in Stat5a/5b fl/fl;Cre compared with Stat5a/5b fl/fl, and that Elf5 mRNA was induced to a greater extent by prolactin in mammary tissue of Stat5a/5b fl/fl mice as compared with Stat5a/5b fl/fl;Cre mice (Supplemental Fig. 4), indicating cell- and stage-specific ... WebHomozygous null mice display embryonic lethality. Embryos homozygous for one null allele failed to develop extraembryonic ectoderm, did not maintain trophoblast stem cells, and had abnormal or absent mesoderm. Mice heterozygous for another allele had impaired lactation and mammary gland development. Gene Ontology. (GO)

WebS. Singh et al. Generation of a new Elf5-Cre BAC mouse model. Results and Discussions Construct design and generation of Elf5CreERT2-GFP mice To develop Elf5CreERT2 … WebApr 19, 2024 · Generation of Elf5 Cre ERT 2-GFP mice. (A) Schematic diagram of the strategy followed to insert Cre-ERT2-GFP gene segment in second exon of Elf5 gene. (B) PCR image showing presence of Cre …

WebThe transcription factor E74-like factor 5 (Elf5) functions downstream of the prolactin receptor signaling pathway and plays an important role in mammary gland development. Using conditional mouse knockouts, we have previously shown that Elf5-null mammary glands exhibit a complete failure of alveolo … http://genesdev.cshlp.org/content/23/20/2382.full

WebMar 28, 2024 · Tg (Elf5-cre/ERT2,-GFP)2Rcha. Mutation details : A partial coding sequence of exon 2 of Elf5 was replaced with CreERT2-P2A-eGFP-SV40pA-Frt-Neo cassette-Frt …

WebPK ÒX‹VÞ ´·8 torchdata/__init__.py]QMK 1 ¼/ì º— uQðTèAk…‚ŠPo"KHÞºÑ4Y_ÒÒý÷&Û´ sËÌ› ¼TXº~`ýÕ Lå ^( ¼ ZÇ[?ÃÚÊ Â*ˆ¶ÕF‹@¾.‹ ÷Æ`Ty0yâ=©„'ê½Ó ÞíX ¤S„x5Z’õ¤°³Š ¡# ÀZÂÃas£6f:CD. ©¯¸ =îÖÛçÃ å0 d ³!$!o,#8 õïœ Qy£†_Ja ¨ëÊ :âÁà ûñÈâ[l’š¤×Á(L' ¢ ²5'_“úFq Û ®p·¨…gGÈËæ›åƒ¤yçbû—X ... eju8582WebJun 17, 2024 · However, after mating of Elf5-Cre; Dcaf2fl/+ mice with Dcaf2fl/fl, no Dcaf2 cKO pups were born. Timed pregnancy studies have shown that Dcaf2 cKO mice developed abnormally on embryonic day 5.5 and ... tead基因名WebThe Elf5-null mice have been described previously [14]. Mice of C57BL/6 strain (6–8 generations) were used for experi-ments with virgin mammary glands. The genotype of the con-trol wild-type (WT) animals was either K14-Cre/Elf5þ/þ or Elf5f/f, whereas the genotype of the Elf5 conditional knock-outs (Elf5-null) was K14-Cre/Elf5f/f. Animal ... teadvuslik eju8573WebOct 3, 2024 · The ablation of Sirt1 protein in Elf5 cre/+, Sirt1 fl/fl mice was first confirmed by IF staining (Figure 5b), and western blotting indicated that compared with those in the placentas of Sirt1 fl/fl littermates, in addition to increased acetylation of P53, the levels of both the P53 and P21 proteins were increased in Elf5 cre/+, Sirt1 fl/fl ... eju8570WebSynonym: Elf5-Cre Molecular description: CRISPR/Cas-mediated genome engineering T2A in front of the Cre coding sequence was inserted into the last coding exon of Elf5, immediately before the stop codon. eju8872http://www.informatics.jax.org/recombinase/specificity?id=MGI:3699244&system=reproductive+system eju8619