Fitc偶联annexin-v凋亡检测试剂盒
http://www.bio-review.com/fitc-labeling-kit/ Web推荐的实验流程. FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10e-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. FITC Annexin V binding is calcium dependent and defined calcium and salt concentrations are required ...
Fitc偶联annexin-v凋亡检测试剂盒
Did you know?
WebProduct overview. Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy. The kit can differentiate apoptosis vs ... WebDocuments. 产品描述. 本试剂盒用于检测细胞凋亡早期的发生,其中Annexin V为胞内蛋白膜联蛋白家族成员,以钙离子依赖的方式选择性与磷脂酰丝氨酸 (PS)结合。. PS正常分布在细胞膜内侧,即与细胞浆相邻的一侧。. 在细胞发生凋亡的早期,不同类型的细胞都会把PS ...
WebAnnexin V是一种磷脂结合蛋白,与磷脂酰丝氨酸有高度亲和力,故可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。. 因此Annexin V被作为检测细胞早期凋亡的灵敏指标之一。. 碘化丙啶(Propidium Iodide, PI)是一种核酸染料, 它不能透过完整的细胞膜 ... WebDec 7, 2024 · FITC偶联标记试剂盒产品特点:. 方便 – 可直接用于偶联抗体或者其它蛋白质. 优化的方案 - 遵循标准实验流程,凭借优异的荧光染料:蛋白质比率,和专利溶液配方,以获得最佳活性和荧光. 一次性包装 – 试剂盒提供的试剂均为单独一次性包装,避免污染. 高效 ...
WebTransfer 100 µL of cell suspension in 5 ml test tube. 3. Add 5 µL of FITC Annexin V. 4. Add 10 µL of PI solution (Cat. No. 421301) or 7-AAD (Cat. No. 420403/420404). 5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 6. Add 400 µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. WebDead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry: Kit Contents: Contains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X …
WebMethod. 1. Cell incubation with Annexin V-FITC. 1.1 Induce apoptosis via the desired method. 1.2 Collect 1–5 x 10 5 cells by centrifugation. 1.3 Resuspend cells in 500 µL of 1X Annexin V binding buffer. 1.4 Add 5 µL of annexin V-FITC and 5 µL of propidium iodide (PI, optional). 1.5 Incubate at room temperature for 5 min in the dark.
Web一般描述. Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞,并使用流式细胞仪进行定量。. AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯(FITC)偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。. 该试剂盒包含碘化丙啶 (PI),用于标记细胞膜完全破坏 … t shirts online bedrucken lassenWebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate. phil reed motorcycleWebThe Annexin V-FITC Kit contains reagents for the simultaneous fluorescent detection of apoptotic, necrotic, and dead cells through the specific binding of FITC-labeled Annexin V to phosphotidylserine (PS). The kit consists of Annexin V-FITC, Annexin V Binding Buffer, and Propidium Iodide Solution. t shirts on harwin in houston texasWeb在细胞凋亡过程中,PS转位到质膜的外叶,当钙存在时,可通过与荧光染料标记的Annexin V结合,通过流式细胞术和细胞成像进行检测。 BD Biosciences提供几种常见形式的Annexin V,如用于紫色激光的FITC、PE、BD Horizon™ BV421和用于紫外激光的BD Horizon™ BUV395。随着这些 ... tshirts on igWebStaining. 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of FITC Annexin V and 5 µl PI. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. phil reed tuckerton njWeb概述. 本试剂盒提供快速而简单的偶联过程,FITC是目前常用的荧光染料之一,通过抗体或蛋白的伯胺(即赖氨酸残基)与FITC的硫碳胺基结合,形成FITC-蛋白质结合物,即FITC标记抗体或蛋白。. 标记抗体或蛋白可应用于ELISA,Western blot,IF和 FACS分析等。. phil reese azphil reese arizona business broker