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H9c2 cell transfection

WebCell Culture and Transfection. Rat cardiomyocytes H9c2 was purchased from the American Type Culture Collection (ATCC, USA), and cultured in DMEM (Thermo, USA) supplemented with 10% FBS (Gibco, USA) ... H9c2 cells were seeded on glass coverslips overnight, followed by fixation and permeabilization by ice-cold 100% methanol for 5 min … WebTransfection of H9C2 cells with 20 M.O.I of GFP-mCherry-LC3 adenovirus for 24 h and then treated with DOX and rapamycin for 24 h and 8 h respectively. Then remove the culture medium, cold methanol fix for 15 min in 4 ℃. After two times washing by PBS, the nuclei were stained by incubating DAPI with the samples for 5 min at room temperature. ...

Ultrasound-targeted microbubble destruction (UTMD) assisted

WebMar 2, 2024 · H9c2 cells were lysed in RIPA lysis buffer (Epizyme Biotech, Shangai, China) and centrifuged at 12,000 ... On the other hand, transfection of miR-31-5p mimic in H9c2 cells led to decreased levels of circPan3 compared with the control (Fig. 4G). Together, these results indicated that miR-31-5p is involved in DOX-induced cardiotoxicity via the ... WebIn this study, we evaluated the efficiency of two transfection methods, chemically mediated polyethyleneimine (PEI) treatment and physically mediated electroporation, on a rat heart … headset macer https://heavenly-enterprises.com

Efficiency of DNA Transfection of Rat Heart Myoblast Cells H9c2 …

WebApr 13, 2024 · H9c2 cells were pretreated with 32 μm Fer-1 for 24 h before synchronization. Samples in an experiment represented one independent replicate, and every experiment needed to be repeated at least 3 times. Cell Transfection The oligonucleotides were transfected into cells according to the manufacturer’s instructions. WebJan 13, 2015 · CELL TRANSFECTION IN HEK-293 and H9c2 (2-1) cell lines In each well of a 6-well plate, seed either HEK-293 or H9c2 (2-1) 5x105 cells in 4ml of complete growth … WebRecommended Transfection Reagents. Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 “It has exceeded my expectations and has worked better than our … headset macbook pro

What are optimal H9C2 seeding and culture conditions?

Category:H9c2(2-1) - CRL-1446 ATCC

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H9c2 cell transfection

Gene Expression Profiling of H9c2 Myoblast Differentiation ... - PLOS

Weba. Change your culture conditions: use an endotoxin and contaminant free medium for culture and complexes formation. Any trace of endotoxin can … WebApr 12, 2024 · After transfection and hydrogen peroxide stimulation, H9C2 cells were fixed with 4% paraformaldehyde and cells were incubated with 50 μg/ml WGA for 30 min at 37 °C. Images were observed by fluorescence microscopy. Surface area was measured with ImageJ and the value was expressed as fold change relative to control.

H9c2 cell transfection

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WebMar 27, 2024 · After transfection for 24 h, H9C2 cells were divided into the following three groups: control, H/R, and BBR+H/R groups. After H/R treatment, the luciferase activities were measured with a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer's instructions. The data were normalized for transfection efficiency by … WebFlow cytometry was employed to detect the apoptosis of H9c2 cells. The interaction between HOTAIR and miR-17-5p was determined by bioinformatics analysis, luciferase reporter gene analysis, and RNA immunoprecipitation experiments. ... Next, after the transfection of HOTAIR siRNA, H9c2 cells were pre-treated with PPF and exposed to …

WebBased on our proteomic analysis, its presence in EVs was linked to hypoxia in HL1 and H9c2 cells, and we hypothesize that EV-associated ENPL may have a cardioprotective role by reducing cardiomyocyte ER stress. ... Transfection of H9c2 and HL1 Cells with pMAXGFP-ENPL Plasmid. H9c2 and HL1 cells were cultured up to 80% confluency in … WebMar 1, 2016 · What is the recommended seeding density for H9C2 cells (specifically in a plastic tc t25 flask)? We have been seeding at the recommended 10x10^4/cm^2 (250,000 in t25) and they are reaching ~70%...

WebJun 29, 2015 · H9c2 myoblasts are a cell model used as an alternative for cardiomyocytes. H9c2 cells have the ability to differentiate towards a cardiac phenotype when the media serum is reduced in the presence of all-trans-retinoic acid (RA), creating multinucleated cells with low proliferative capacity. In the present study, we performed for the first time … WebThe H9c2 cell line was cultivated in DMEM contained with 10% (v/v) FBS, 100 U/ml penicillin and 100 µg/ml streptomycin in a 37 °C environment with 5% CO 2. To simulate the myocardial ischaemia, H9c2 cells were handled with hypoxia. H9c2 cells were incubated in hypoxic incubator containing 94% N 2, 5% CO 2 and 1% O 2 to simulate hypoxia for 24 h.

WebApr 9, 2024 · Cell transfection. H9C2 cells were transfected with HIF-1α siRNA, GL2 siRNA (Thermo Fisher Scientific), miR-711 mimics, miR-711 inhibitors or their relative controls (Qiagen) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) or Endofectin (GeneCopoeia, Rockville, MD) to knock down HIF-1α and miR-711, or over …

WebLipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to ... headset malaysiaWebApr 9, 2024 · H9C2 cells were transfected with HIF-1α siRNA, GL2 siRNA (Thermo Fisher Scientific), miR-711 mimics, miR-711 inhibitors or their relative controls (Qiagen) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) or Endofectin (GeneCopoeia, Rockville, MD) to knock down HIF-1α and miR-711, or over-express miR … gold toe knee high socks womenWebH9c2 Transfection Reagent (Embryonic Myocardium) $ 326.00 – $ 1,794.00 SKU: H9C2 Product Availability: In Stock (FedEx delivery within 2 business days). Ordering: To place an order please use Add to Cart … headset making static sound