WebCell Culture and Transfection. Rat cardiomyocytes H9c2 was purchased from the American Type Culture Collection (ATCC, USA), and cultured in DMEM (Thermo, USA) supplemented with 10% FBS (Gibco, USA) ... H9c2 cells were seeded on glass coverslips overnight, followed by fixation and permeabilization by ice-cold 100% methanol for 5 min … WebTransfection of H9C2 cells with 20 M.O.I of GFP-mCherry-LC3 adenovirus for 24 h and then treated with DOX and rapamycin for 24 h and 8 h respectively. Then remove the culture medium, cold methanol fix for 15 min in 4 ℃. After two times washing by PBS, the nuclei were stained by incubating DAPI with the samples for 5 min at room temperature. ...
Ultrasound-targeted microbubble destruction (UTMD) assisted
WebMar 2, 2024 · H9c2 cells were lysed in RIPA lysis buffer (Epizyme Biotech, Shangai, China) and centrifuged at 12,000 ... On the other hand, transfection of miR-31-5p mimic in H9c2 cells led to decreased levels of circPan3 compared with the control (Fig. 4G). Together, these results indicated that miR-31-5p is involved in DOX-induced cardiotoxicity via the ... WebIn this study, we evaluated the efficiency of two transfection methods, chemically mediated polyethyleneimine (PEI) treatment and physically mediated electroporation, on a rat heart … headset macer
Efficiency of DNA Transfection of Rat Heart Myoblast Cells H9c2 …
WebApr 13, 2024 · H9c2 cells were pretreated with 32 μm Fer-1 for 24 h before synchronization. Samples in an experiment represented one independent replicate, and every experiment needed to be repeated at least 3 times. Cell Transfection The oligonucleotides were transfected into cells according to the manufacturer’s instructions. WebJan 13, 2015 · CELL TRANSFECTION IN HEK-293 and H9c2 (2-1) cell lines In each well of a 6-well plate, seed either HEK-293 or H9c2 (2-1) 5x105 cells in 4ml of complete growth … WebRecommended Transfection Reagents. Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 “It has exceeded my expectations and has worked better than our … headset macbook pro