WebbThis sets up the OGT/OGA pair to be an “all-in-one” metabolic and nutrient sensor and has been understood to alter diverse cellular processes such as apoptosis, gluconeogenesis, calcium signaling, insulin signaling, and … Webb27 okt. 2024 · Specific bands were visualized using an enhanced chemiluminescence detection kit (ECL kit; Millipore, Billerica, MA). ... OGT is highly expressed in aggressive prostate cancer, and high OGT activity is essential for a transcription factor MYC-driven proliferation of prostate cancer cells (Itkonen et al., 2024).

Research Paper High OGT activity is essential for MYC-driven

Webb9 aug. 2024 · O-GlcNAc transferase (OGT) is the sole enzyme transferring N-acetylglucosamine from uridine diphosphate (UDP)-GlcNAc to selected serine/threonine residues of cytoplasm and nucleus proteins. Aberrant of OGT activity is associated with several diseases, suggesting OGT as a novel therapeutic target. Webb24 apr. 2024 · Phosphoproteome changes implicate OGT activity in RNA splicing. (A) Structure of OSMI-2.(B) Relative protein O-GlcNAc levels in HEK-293T cells show substantial changes by 0.5 h treatment with OSMI-2 (see also Supplementary Figure S1A).(C) Scheme depicting treatment conditions for quantitative … how to add items to list java

High OGT activity is essential for MYC-driven proliferation of …

Webb21 mars 2024 · OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase) is a Protein Coding gene. Diseases associated with OGT include Intellectual Developmental … Webb22 aug. 2012 · OGT and OGA Activity Assays The protein pellets were resuspended in the OGT assay buffer (25 mM HEPES, pH 7.5, 10 mM MgCl 2 and 1 mM EDTA) and centrifuged at 16000 g at 4°C for 20 sec. The final pellets were resuspended in 150 µl of 0.5% SDS, followed by bath sonication for 5 min. WebbComposite structure from PDB structures XXXX and XXXX. OGT is a proposed target for treating cancers and cardiometabolic disorders. Our goals are to understand OGT’s … methodized learning sat