Webb根据pHIS2载体上 Eco R Ⅰ和 Sac Ⅰ限制性内切酶识别位点的前后序列设计载体引物，进行PCR菌液检测，以阳性菌液为模板的PCR扩增的DNA条带要比以pHIS2空载体为模板的PCR扩增的条带分子量大20个碱基左右。 从 图 2 中可以看出，泳道1-13、15-18、19、21、23-26、28-30、32-37、40、41和43-48号的PCR扩增条带比对照分子量大，初步证明这些 … WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: …
所有质粒载体汇总情况 - 道客巴巴
Webb一、转化诱饵载体（参考Super转化试剂盒SK2402质粒转化步骤） 1.YPDA平板培养划线，活化Y187酵母菌种；挑酵母菌落于液体YPDA培养基摇菌培养； 2.制备感受态并 … http://www.vectorhub.cn/difference-between-plasmid-vector.html eai winner ford
phis2酵母表达载体说明 - 豆丁网
WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb1 载体构建 根据客户提供基因序列构建好的pBait-AbAi载体 2 pBait-AbAi质粒线性化 图1. pBait-AbAi BstBI酶切 Lane M：DNA Marker 1kb Lane 1：酶切前p53-AbAi Lane 2：酶切后p53-AbAi Lane 3：酶切前pAbAi Lane 4：酶切后pAbAi Lane 5：酶切前pBait-AbAi Lane 6：酶切后pBait-AbAi 由图1可知，p53-AbAi质粒、pAbAi质粒和pBait-AbAi质粒均已正确 … Webb载体描述 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). e a its in the game