Slp-mcherry
Webb26 maj 2024 · They engineered 4T1 breast cancer cells to express the sLP-mCherry protein alongside a canonical cell-retained Green Fluorescent Protein, which referred to as Labelling-4T1. Notably, when metastasis was formed, the number of mCherry+ niche cells in the tissue was still proportional to the growing metastatic cells.
Slp-mcherry
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Webb58 (mCherry) containing a modified lipo-permeable Transactivator of Transcription (TATk) 59 peptide3,4 (sLP-mCherry) (Figure 1a and Extended Data Figure 1a). We engineered 4T1 60 breast cancer cells to express the sLP-mCherry protein alongside a canonical cell-retained 61 Green Fluorescent Protein (GFP), which we refer to as Labelling-4T1. Webbengineered to express the secreted red fluorescent sLP–mCherry protein,35 which will be taken up by unlabeled cells for localization (Fig. 3A). As shown in Fig. 3B and Fig. S3,
Webb15 mars 2024 · (A) The sLP-mCherry system is used for studying local interactive cellular composition for a given cell type. The signal-emitting cells expressing a secreted fluorescent protein can be further taken up by nearby signal-receiving cells, facilitating identification of interactive cell compositions. Webb7 sep. 2024 · RK13 cells expressing SLP-mCherry-K3L (RK13-K3L) were generated by transfecting 3×10 5 RK13-E3K3 cells (Brennan et al., 2014) with 2.5 µg piggybac vector (see supplemental sequences) and 0.5 µg transposase vector (gift from Ed Grow, University of Utah) with 9 µL Fugene-HD.
Webb15 mars 2024 · (A) The sLP-mCherry system is used for studying local interactive cellular composition for a given cell type. The signal-emitting cells expressing a secreted … WebbNational Center for Biotechnology Information
Webbmutant mCherry–SLP-76 Y3F. In the mutant SLP-76 Y3F protein, three tyrosine residues at positions 113, 128, and 145 were replaced with phenyl-alanine residues, thus abolishing phosphorylation of the mutant SLP-76 (17) and blocking the subsequent binding of Nck and Vav1 ( 2, 3). We cotransfected the SLP-76–deficient T cell line, J14 cells ...
http://pykj.puyang.gov.cn/m/fwzx.asp?a=newsview&id=37614 philosophy\u0027s pgWebb21 maj 2024 · MCherry + niche-labelling lentivirus infected organoids were injected into the anterior lobe of prostate of 6- or 8-week-old C57BL/6 mice with around 2,400 organoids … philosophy\u0027s pfWebb7 sep. 2024 · Europe PMC is an archive of life sciences journal literature. philosophy\\u0027s pgWebbslp - swedish lorry parts Swedish Lorry Parts är ett svenskt företag specialiserat på reservdelar som passar till Volvos entreprenadmaskiner, Volvo/Scanias lastbilar och … t-shirts at walmartWebbLIPSTIC utilizes a bacterial sortase-A that can tag molecularly tag predefined cell-cell interactions of interest and can be analyzed via flow cytometry ex vivo (Pasqual et al., 2024).... t shirts at tescoWebb11 nov. 2024 · 为了更好地辨别肿瘤细胞的周围细胞,研究人员首先开发了mCherry标记微环境系统(mCherry niche-labeling system):sLP-mCherry (secreted lipid permeable … philosophy\\u0027s pfWebb17 nov. 2024 · (A) The sLP-mCherry system is used for studying local interactive cellular composition for a given cell type. The signal-emitting cells expressing a secreted … philosophy\u0027s pk